| 1. | Abstract : techniques of apple genetic transformation can improve apple trees and shorten the breeding cycle by molecular methods . in the last years , great progress has been made in this field , which involves a number of important apple genotypes developed . so far , apple genetic ransformation mainly adopts agrobacterium - mediated technique , involving infection and transgenic tissue regeneration , which are important steps to affect the transformation efficiency . the initial work to the date is to know and to search for the factors which can increase the efficiency 摘要苹果的遗传转化技术通过分子手段改良苹果,有助于缩短其育种周期.最近十年,在该领域的研究取得很大进展,涉及到一些重要的苹果基因型及有用的外源基因.迄今,苹果的遗传转化主要采用农杆菌介导法,侵染与转化材料的再生是影响其转化效率的关键过程,了解其影响因素,寻找有利因素以提高转化效率是目前苹果遗传转化研究的重点。 |